Lasers Promote Faster, Stronger Wound Closure

Through increased cellular oxygenation and metabolism, lasers promote fibroblast proliferation, collagen regulation and a host of positive enzymatic changes.


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Wounds and dermatology issues represent perhaps the widest variety of ailments in veterinary practice, both in origin and complication.

From an acute burn to a chronic lick granuloma, from an ischemic ulcer to a dehiscent surgical site, no two wounds will be alike. Compound this variation with different histologies and enzyme over/under-expressions; then throw bacterial or fungal infections on top of all that; now plan for a dog gnawing at it constantly or a horse sleeping in a dirty stable.

Snake Bite
This is a snake bite case handled by someone who was skeptical about therapeutic lasers. The dog was bitten in the hind limb and after three days, the entire belly was necrotic. Veterinarians did a full surgical debridement on Day 5 and one day later, the necrotic tissue returned to the entire area. At this point on Day 6 they applied the laser, but in the interest of science and skepticism, they left a control section, lasering two-thirds of the belly and leaving one-third untouched, above right. Two days and two treatments later, the effects on each section are clear. At this point, based on the immediate results, they abandoned the control area and treated the entire belly once more. As of Day 12, the two-thirds section has had three laser treatments and the one-third section has had 1. Remember how quickly the necrosis spread after the initial debridement? Notice that this is a very clean wound even six days after that debridement. This wound remains clean and the infection has been neutralized.

Laser therapy on snake bite
Before treatment

Laser therapy
Area at far right was not initially lasered.Click to enlarge.

Needless to say, having one modality that functions well throughout this gauntlet of variation is invaluable. Laser therapy has documented success treating each corner of this wound healing domain, with particular success (over other modalities) in contaminated wounds and infections.

Through increased cellular oxygenation and metabolism, lasers promote fibroblast proliferation, collagen regulation and a host of positive enzymatic changes that lead to faster and stronger wound closure. Simultaneously bringing more oxygen to anaerobic fungi and bacteria, lasers can provide anti-microbial protection.

Unfortunately, the job is not finished when the wound has been disinfected and closed. Scarring, both internal and external, can lead to a host of problems for the animal.
 
In joints, for example, a post-surgical scar can lead to limited range of motion as well as chronic arthritis. A scar on an internal organ can affect the functional recovery. Fortunately, the fundamental nature of laser therapy can reduce this risk.

At the core of every wound there is a region that has a very high concentration of collagen, through which there is minimal blood flow. After a time, the fibrotic cross-linking of this collagen matrix denies any nutrients to the central cells, and they become dormant, never to be fully healed. Laser therapy counteracts this process in two ways.

First, laser will increase the micro-circulation via angiogenesis through the matrix, vasodilation and increased overall blood perfusion. Dozens of papers have demonstrated this effect, some through histology reports of mast cell degranulation and prostaglandin increases, others through thermography and laser speckle flowmetry, but most notably Kubota (Lasers Med Sci 2002, 17:146–153) and Uozumi et al. (Lasers Surg Med 2010, 42:566–576). These papers, among others, have shown an immediate as well as a lasting increase in localized blood flow.

With this increase of blood flow and nitric oxide concentration comes a subsequent increase in cellular oxygenation. This phenomenon alone is well known to speed up the wound closure and repair processes (in fact, the entire negative-pressure modality is based on this), but laser therapy goes one step further.

There is profound evidence that laser’s interaction with cytochrome oxidase in the mitochondria leads to more rapid redox cycle and ultimately an increase of ATP synthesis (Karu et al. Photomed Las Surg 2008, 26, 6:593-599). Once the cell is introduced to and has burned its oxygen-fuel into chemical energy, all of the cellular compartments will begin to function, and the cells that were previously nutrient-poor and dormant will self-regulate their collagen, reducing the breadth and rigidity of the scar.

Of course other modalities are specifically suited to aid in the healing process of a particular type of wound based on the histological and enzymatic profile of a given patient. But the fundamental action of laser therapy makes it perhaps the most versatile piece of equipment a general practitioner can have. Neither the origin of nor the complications that arise from any wound a veterinarian faces (malignant cancers aside) will exclude the potential benefits of laser therapy.

For a full bibliography of case histories, fundamental mechanism papers and literature metareviews of wound healing and other applications of laser therapy in veterinary medicine, contact the author directly at bstephens@k-laserusa.com.

Bryan J. Stephens, Ph.D., is the director of research and development for K-Laser USA. He is an expert in radiation’s interaction with biological matter, specifically in radiation dosimetry and photobiology.

This Education Series article was underwritten by K-Laser USA of Franklin, Tenn.

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