Cotton Rope Makes Collecting Diagnostic Samples from Pigs Easier

Iowa State University veterinarians have found that taking oral fluid samples from a rope that pigs have chewed on allows them to monitor diseases accurately without the difficult step of taking individual blood samples.

Dr. Jeff Zimmerman

Iowa State University

Veterinarians at Iowa State University have found an easier way to collect diagnostic samples from pigs: by collecting oral fluid samples from a rope that the pigs have chewed on.

Taking individual blood samples from pigs to monitor infections imposes costs and time constraints on veterinarians and pork producers, according to Jeff Zimmerman, DVM, Ph.D., Dipl. ACVPM, a professor of veterinary diagnostic and production animal medicine at Iowa State University.

“The pigs aren’t happy about collecting blood samples, and neither are the people,” Dr. Zimmerman said.

He and some of his colleagues began their experiments to find an easier means of collecting samples by hanging ropes in pens of pigs. The pigs enthusiastically chewed on the rope, leaving an oral fluid sample in the process.

The researchers began the project in 2005 as part of their work on porcine reproductive and respiratory syndrome (PRRS) virus. Pigs instinctively chew on objects, including ropes hanging in their pen, as a means of exploring their surroundings, Zimmerman said. But the researchers didn’t know if the oral fluid samples left on the rope would allow them to track the infections they were interested in monitoring.

As they refined the process, they learned that oral fluid samples can lead to diagnostic accuracy as good as—and usually better than—conventional surveillance techniques, according to Iowa State University.

Oral fluid samples collected through this method can help veterinarians detect a wide variety of infections, including foot and mouth disease and classical swine fever, Zimmerman said.

The technique is gaining wide acceptance, according to the university. The Iowa State University Veterinary Diagnostic Laboratory ran 10,268 oral fluid tests in 2010, but by 2015, that total jumped to 176,167. Producers in Europe, the Americas and Asia have also begun to collect oral fluid samples, the university further noted.

Testing oral samples saves producers money and avoids the difficulty of taking individual blood samples from pigs, Zimmerman said. He estimated the cost of testing for PRRS is between 3 cents to 12 cents per pig in the barn, depending on the kind of test used. By contrast, the cost of a PRRS virus outbreak in growing pigs can cost between $7 and $15 per pig, according to Zimmerman.

The researchers have tested cotton, nylon and hemp ropes and found that cotton produces the best testing results.

Growing pigs four weeks or older require no training for oral fluid collection, Zimmerman said. The rope proves irresistible to them due to their natural curiosity. For younger pigs, Zimmerman suggested that a rope be left on the floor of their pen for 30 minutes to allow the piglets to grow accustomed to it in a nonthreatening way before trying to collect samples.

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